P-233 Novel, non-invasive imaging techniques in quality assessment of mammalian eggs

Abstract Study question Can Optical Coherence Microscopy (OCM) imaging and Cytoplasmic Movement Velocity (CMV) analysis be useful in quality assessment of mature mammalian oocytes? Summary answer OCM allows for oocytes’ metaphase spindles, CMV – the actomyosin cytoskeleton functionality, therefore they are potentially oocyte selection IVF. What is known already The success rate IVF has significantly improved over recent years, yet its efficiency plummets with increasing age female patients. Therefore, novel approaches to oocyte/embryo high demand. Among most promising new methods OCM, a label-free, 3D tool subcellular structure visualization, such as meiotic spindle oocytes or nuclear immature oocytes, CMV, time-lapse imaging-based method studying properties cytoskeleton, crucial plethora cellular process. Both these provide unique insights into competence. design, size, duration MII dimensions were measured OCM-imaged freshly ovulated collected from young mice (FO-MII, n = 108) correlated outcome preimplantation development achieved after activation. Spindles FO-MII (n 45) also compared spindles advanced maternal (AMA-MII, 44). was imaged 24), postovulatory aged (PA-MII, 16), AMA-MII 19). Participants/materials, setting, Oocytes hormonally primed using customized protocol. They then activated parthenogenetically cultured 5 days, followed by immunofluorescence staining assess total number cells first embryonic cell lineages. FO-MII, PA-MII, (6 frames per minute; 15 min). images subjected particle image velocimetry mean calculated. Main results role chance that blastocyst stage had smaller volume (p < 0.001) width 0.05) those failing do so. Univariate logistic regression indicated 1 μm rise decreases odds formation 28% on average 8, whereas 100 μm3 almost 10% 2.7 respectively. Moreover, related trophectodermal cells, length - primitive endoderm blastocysts. Noteworthily, 0.0001), well narrower 0.0001) their counterparts females. A multivariate linear model combining three morphokinetic parameters (t3, cc2b, tSB) explained 60% variability 5-day-old embryos (R2 0.604) while models based solely single only up approx. 54% 0.537, tSB). On other hand, revealed eggs display faster cytoplasmic movements than postovulatory-aged 0.0001). Reduced speed observed Limitations, reasons caution All studies presented here performed mouse model, so obtained data needs verified target species validation tools protocols. Wider implications findings Our suggest OCM-based short, 15-minute allow higher could find application assisted reproductive technologies, non-invasive may enhance universally acclaimed Trial registration not applicable